CIE A Level Biology复习笔记3.2.2 Rate: pH
Rate: pH All enzymes have an optimum pH or a pH at which they operate best Enzymes are denatured at extremes of pH Hydrogen and ionic bonds hold the tertiary structure of the protein (ie. the enzym...
Rate: pH All enzymes have an optimum pH or a pH at which they operate best Enzymes are denatured at extremes of pH Hydrogen and ionic bonds hold the tertiary structure of the protein (ie. the enzym...
Rate: Temperature Enzymes have a specific optimum temperature – the temperature at which they catalyse a reaction at the maximum rate Lower temperatures either prevent reactions from proceeding or ...
Colorimetry to Measure Enzyme Activity A colorimeter is able to measure light absorbance (how much light is absorbed) or light transmission (how much light passes through) a substance Colorimetry c...
Measuring Enzyme Activity The progress of enzyme-catalysed reactions can be investigated by: Measuring the rate of formation of a product using catalase Measuring the rate of disappearance of a sub...
How Enzymes Work The lock-and-key hypothesis Enzymes are globular proteins This means their shape (as well as the shape of the active site of an enzyme) is determined by the complex tertiary struct...
Mode of Enzyme Action Enzymes have an active site where specific substrates bind forming an enzyme-substrate complex The active site of an enzyme has a specific shape to fit a specific substrate Ex...
Enzymes as Proteins Enzymes are biological catalysts ‘Biological’ because they function in living systems ‘Catalysts’ because they speed up the rate of chemical reactions without being used up or c...
Water Molecules: In Living Organisms Water has many essential roles in living organisms due to its properties: The polarity of water molecules The presence and number of hydrogen bonds between wate...
Water Molecules: Hydrogen Bonds Water is of great biological importance. It is the medium in which all metabolic reactions take place in cells. Between 70% to 95% of the mass of a cell is water As ...
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